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28 People often continue to experience mild neurological symptoms such as poor memory, impaired concentration, and fatigue even after “adequate” B 12 replacement (eg, therapy according to the BNF suggested treatment regimen) 9 17 29 and many patients report they need additional treatment

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Quantitative Real-Time PCR (qRT-PCR) was used to measure mRNA expression of SASP factors (IL-1β, IL-6, TNF-α, TGF-β) in TM3 cells, normalizing data to GAPDH